Open AccessHypermethylation of Tumor-related Genes in Genitourinary Cancer Cell Lines
Author(s) -
Woon Bok Chung,
Su Hyung Hong,
Jin A Kim,
Yoon Kyung Sohn,
Bup Wan Kim,
Jung Wan Kim
Publication year - 2001
Publication title -
journal of korean medical science/journal of korean medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.743
H-Index - 66
eISSN - 1598-6357
pISSN - 1011-8934
DOI - 10.3346/jkms.2001.16.6.756
Subject(s) - dna methylation , methylation , cancer research , biology , cpg site , cancer , gene , gstp1 , prostate cancer , microbiology and biotechnology , genetics , gene expression , genotype
Hypermethylation of CpG island is a common mechanism for the inactivation of tumor-related genes. In the present study, we analyzed 13 genitourinary cancer cell lines for aberrant DNA methylation of 5 tumor-related genes using methylation- specific polymerase chain reaction (MSP). GSTP1 was methylated in 5 (38.5%), E-cadherin in 1 (8%), VHL in 1 (8%), and MGMT and hMLH1 in none (0%). Six out of thirteen genitourinary cancer cell lines had methylation of at least one of five genes; 5 had one gene methylated, and, 1 had two genes methylated. Methylation of these 5 genes was not detected in any of the bladder cancer cell lines. GSTP1 was methylated in all of the 3 prostate cancer cell lines. We conclude that aberrant hypermethylation may be an important mechanism for the inactivation of cancer-related genes in kidney and prostate cancer cell lines.