Comparison between Real-Time PCR and Agarose Gel Electrophoresis for DNA Quantification | Zendy

MiKyung Lee | Zendy; Hye-Ryoun Kim | Zendy

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Comparison between Real-Time PCR and Agarose Gel Electrophoresis for DNA Quantification

Author(s) -

MiKyung Lee,

Hye-Ryoun Kim

Publication year - 2006

Publication title -

annals of laboratory medicine

Language(s) - English

Resource type - Journals

eISSN - 2234-3814

pISSN - 2234-3806

DOI - 10.3343/kjlm.2006.26.3.217

Subject(s) - ethidium bromide , agarose , agarose gel electrophoresis , real time polymerase chain reaction , microbiology and biotechnology , polymerase chain reaction , gel electrophoresis , chromatography , electrophoresis , molecular weight size marker , primer dimer , gel electrophoresis of nucleic acids , polymerase chain reaction optimization , biology , chemistry , dna , gel electrophoresis of proteins , multiplex polymerase chain reaction , polyacrylamide gel electrophoresis , gene , genetics , biochemistry , enzyme

Real-time polymerase chain reaction (PCR) is generally regarded as a very accurate and time-saving method, but it is expensive to run. We evaluated the reliability of an inexpensive and a researcher-friendly gel electrophoresis-based PCR method for the quantification of mRNA, and the results were compared with those obtained by real-time PCR.

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