INDUKSI KALUS STEVIA REBAUDIANA BERTONI M. DENGAN PEMBERIAN KOMBINASI ZPT NAA (NAPHTALENE ASETIC ACID), 2,4-D (2,4 DICLOROPHENOXY ASETIC ACID) DAN BAP (BENZIL AMINO PURIN)

Stevia is a plant that produces natural sweetener with sweetness level about 300 times higher than sugar. This sweetener was obtained mainly in the leaf of stevia, which contains stevioside and rebaudioside-A (diterpen glycosides). Production of stevioside can be generated in callus from leaves of Stevia through the technique of plant tissue culture. The objectives of this research were to induce proliferation of callus and increasing the content of stevioside in callus. This research was conducted in the laboratory of Biotechnology, Faculty of Biology, UGM from June 2015. The design of the research was CRD. Callus production using combination of ZPT Auxin; NAA (2 mg/L) and 2,4-D (2 mg/L) and Cytokinin; BAP (0; 0.5; 1 mg/L). All treatment with 3 replication. Qualitative data such as colour and texture of callus was analyzed as describtive data. The result showed that the combination of 2 mg/L 2,4-D and 1 mg/L is the best concentration to produce callus from leaves of Stevia rebaudiana, indicated by the highest rate of callus induction within 12 days, white color of callus, and friable texture of callus.