AÇÃO PROTETORA DO TROLOX FRENTE AO DANO OXIDATIVO INDUZIDO PELO GLIFOSATO E TROP® EM MODELO ANIMAL

O glifosato e um herbicida utilizado em varios tipos de culturas e e considerado de baixa toxicidade, no entanto, os mecanismos de toxicidade das formulacoes ainda nao estao bem esclarecidos. O objetivo do estudo foi avaliar os efeitos do Trop® e do glifosato sobre os parâmetros oxidativos, potencial genotoxico, funcao renal e  hepatica, em animais submetidos ou nao ao tratamento com vitamina E. Utilizou-se camundongos Swiss machos albinos, divididos em nove grupos (n=8): Controle (salina); glifosato (50 e 500 mg/kg); Trop ® (50 e 500 mg/kg); Glifosato e vitamina E (20 e 200 mg/kg); Trop ® e vitamina E (20 e 200 mg/kg). O tratamento foi realizado em dose unica via oral. Apos 48 horas, os animais foram sacrificados o sangue e o figado coletados. O potencial genotoxico foi avaliado pela tecnica de micronucleos, a peroxidacao lipidica atraves das especies reativas ao acido tiobarbiturico (TBARS); a capacidade antioxidante pela concentracao de glutationa reduzida (GSH) e catalase (CAT); a funcao renal atraves das dosagens de ureia e creatinina e a funcao hepatica atraves das dosagens da Alanina-amino-transferase (ALT) e Aspartato-amino-transferase (AST). Os resultados mostram que nao houve um aumento no numero de micronucleos nos eritrocitos demonstrando assim que nao houve danos no DNA. Os niveis de TBARS e atividade da CAT teve aumento significativo em comparacao aos animais controle e reducao da GSH. Os valores de ureia e creatinina tiveram um aumento significativo quando os animais foram tratados com as doses de 500mg/Kg, no entanto, estes efeitos foram revertidos quando os animais foram tratados com combinacao de vitamina E 200 mg/Kg. As dosagens de AST e ALT nao evidenciaram alteracoes entre os grupos tratados. Os resultados mostram que uma exposicao unica ao glifosato pode causar dano oxidativo hepatico e alteracao na funcao renal que pode ser revertido pela administracao de anti-oxidantes em particular a vitamina E. Palavras-chave : Glifosato. Dano Oxidativo. Potencial Genotoxico. TROLOX OF PROTECTIVE ACTION FRONT OXIDATIVE DAMAGE INDUCED BY GLYPHOSATE AND TROP ® IN ANIMAL MODEL ABSTRACT: Glyphosate is an herbicide used in various types of cultures, considered to have low toxicity, however, the mechanisms of toxicity of the formulations are not wellunderstood. Theaim of the study was to evaluate the effects of Glyphosate on Trop ® and oxidative parameters, potential genotoxic an drenal function in animals submitted or not to treatment with vitamin E. Male albino Swiss mice were used divided into nine groups (n=8). The control group (saline); Glyphosate (50 and 500mg/kg); Trop ® (50 and 500mg/kg); Glyphosate + Vitamin E (20 and 200 mg/kg); Trop® + Vitamin E (20 and 200 mg/kg). Treatment was performed in a single oral dose, 48 hours after treatment the animals were sacrificed the blood and liver were collected. The genotoxic potential was assessed by micronucleus technique. The evaluation of lipid peroxidation was accomplished by determination of thiobarbituric acid reactive species (TBARS). The antioxidant status was evaluated by concentration of reduced glutathione (GSH) and the catalase (CAT) activity. The renal function was evaluated through the urea and creatinine dosages. The results show that there was not increase in the number of micronuclei in erythrocytes of the peripheral blood of animals treated with Glyphosate or Trop ® thus demonstrating that DNA damage has not occurred when animals were treated with the herbicide. However, the oxidative parameters were altered, the levels of TBARS and CAT activity had a significant increase compared to control animals and significantly reduced GSH. The values of urea and creatinine were significantly higher when animals were treated with doses of 500mg/Kg, however, these effects were reversed when the animals were treated with combination of vitamin E 200mg/Kg. The results show that even a single exposure to Glyphosate may cause hepatic oxidative and renal damage which can be reversed by the administration of antioxidants in particular vitamin E. Keywords: Glyphosate. Oxidative damage. Genotoxic potential.