NPR1 gene transformation as assessed by germ cell in situ transformation pathway into Siraitia grosvenorii | Zendy

Jingjian Li | Zendy; Xin Liu | Zendy; Bo Zhao | Zendy; Wenlan Li | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

NPR1 gene transformation as assessed by germ cell in situ transformation pathway into Siraitia grosvenorii

Author(s) -

Jingjian Li,

Xin Liu,

Bo Zhao,

Wenlan Li

Publication year - 2018

Publication title -

bangladesh journal of botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.152

H-Index - 17

eISSN - 2079-9926

pISSN - 0253-5416

DOI - 10.3329/bjb.v44i2.38513

Subject(s) - transgene , transformation (genetics) , biology , genetically modified crops , cauliflower mosaic virus , gene , virus , inoculation , virology , microbiology and biotechnology , genetics , horticulture

NPR1 gene was transformed into Siraitia grosvenorii (Swingle) C. Jeffrey ex. A.M. Lu and Zhi Y. Zhang by germ cell in situ transformation. Ovary injection, cutting chapiter, and chapiter spreading treatments were applied in this study. The transgenic plants were selected using hygromycin screening and confirmed by PCR testing, genome integrated with NPR1 gene in transgenic plants was analyzed by Southern hybridization. Results showed that three treatments could produce transgenic plants. Some of the transgenic plants were selected for tobacco mosaic virus inoculation testing, which showed a higher level of resistance to tobacco mosaic virus than non-transgenic controls.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research