Isolation and Identification of Free Radical Scavenging Compound from Stem Bark Soursoup (Annona muricata L.) Extract

Isolation and Identification of free radical active compound from stem bark soursoup (Annona muricata L .) extract. The aim of this research is to observe the chemical compound of stem bark the soursoup (A. muricata L .) which is active as a free radical. 550 gram of stem bark the soursoup (A. muricata L .) was by graduate extracted using n-heksan, ethyl acetat and ethanol solvents respectively. Extract result is the extraction, it was produced 2.4 gram n-heksan extract, 8.4 gram ethyl acetat extract, 2.1 gram ethanolic extract. The isolation of ethyl acetat extract was conducted using colom chromatography (TLC) method using eluent n-hexane : ethyl acetat (7:3) and it was produced four fractions. The third fraction was isolated using preparative thin layer chromatography with mob ile phase n-hexane : ethyl acetat (6:1) and got 2 bands. The isolate identified by UV-Vis spectroscopy, IR spectroscopy and analyzed by chemistry reaction. From the data result show that isolate is acetogenin.