Screening of blue crab (Portunus pelagicus L.) eyestalk extracts for growth inhibition of Pectobacterium carotovorum subsp. carotovorum (Hauber et at. 1998) (Erwinia carotovora) Jones (Holland) and Raistonia solanacearum (Yabuuchi et al. 1995) (Pseudomonas solanacearum) E. F. Smith
Author(s) -
Julissah Evangelio,
Bernardita Germano
Publication year - 2006
Publication title -
annals of tropical research/annals of tropical research (visayas state university-online)
Language(s) - English
Resource type - Journals
eISSN - 0116-0710
pISSN - 2704-3541
DOI - 10.32945/atr2826.2006
Subject(s) - portunus pelagicus , biology , pectobacterium carotovorum , microbiology and biotechnology , food science , serial dilution , botany , fishery , crustacean , pathogen , medicine , alternative medicine , pathology
Crustacean eyestalks are known to contain neurosecretory hormones that inhibit molting, growth and gonadal development. Along this framework, this study was conducted to: screen crude eyestalk extracts from the blue crab Portunus pelagicus for possible growth inhibition on two bacterial pathogens Ralstonia solanacearum and Pectobacterium carotovorum subsp. carotovorum. and determine effective concentrations of the crude extract against the pathogens. The screening was done through bioassay using the top agar inoculation technique with 70% ethanol as the extracting solvent and streptomycin as the positive control. Different concentrations of blue crab extract and streptomycin were obtained using serial dilution.
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