Use of Some Plants Color as Alternative Staining of Bacteria

Natural dyes from plants such as Stigma (Isatis sp.), Myrtle (Myrtussp.), Rosella (Hibiscussp.) and crust of Walnut (Juglanssp.) fruits were extracted by 95% ethyl alcohol or distilled water. Myrtle and Stigma weremixed dye with ratio 1:2, respectively, also Rosellaand crust of nut fruits were prepared with same ratio. Mixeddyes or stains prepared as alternative of Gram stainfor staining Gram negative and Gram positive bacteria. The results showedthat the wall of bacteria were stained. This is well comparable to Gram stain in respect to clarity, differentiation, and economic cost.