ALANINE AMINO TRANSFERASE (ALT) SPECIFIC ACTIVITIES IN LONG TERM SYSTEMIC HYPOXIC RAT BRAIN TISSUES | Zendy

Rizka Ramadhani | Zendy; Ani Retno Prijanti | Zendy

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ALANINE AMINO TRANSFERASE (ALT) SPECIFIC ACTIVITIES IN LONG TERM SYSTEMIC HYPOXIC RAT BRAIN TISSUES

Author(s) -

Rizka Ramadhani,

Ani Retno Prijanti

Publication year - 2019

Publication title -

acta biochimica indonesiana

Language(s) - English

Resource type - Journals

eISSN - 2654-6108

pISSN - 2654-3222

DOI - 10.32889/actabioina.v2i2.43

Subject(s) - alanine , gluconeogenesis , alanine transaminase , lactate dehydrogenase , transaminase , hypoxia (environmental) , aspartate transaminase , medicine , glutamate receptor , transferase , alanine aminotransferase , endocrinology , biochemistry , amino acid , biology , chemistry , enzyme , alkaline phosphatase , oxygen , receptor , organic chemistry

Background: Brain as a very aerobic organ is sensitive to hypoxia. Energy scarcities must be overcome by gluconeogenesis, which uses alanine or lactate as starting material. The reaction is catalyzed by alanine amino transaminase (ALAT or ALT), also known as glutamate pyruvate transaminase (GPT). Objective : To investigate whether the specific activities of alanine aminotransferase (ALT) increased in hypoxic rat brain. Methods : This experimental study used rats exposed to systemic normobaric hypoxia during 14 days. A group of 5 rats was sacrificed in days 1, 3, 7 and 14. The specific activities of ALT were analyzed in their brains using a reaction coupled with lactate dehydrogenase (LDH) activities. Results : The ALT specific activities in rat brain were very low. There was no significant increase of specific activities during long term hypoxia (p > 0.05). Conclusion : The rat brain ALT has no role in gluconeogenesis.

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