Comparison of 12 DNA extraction kits for vertebrate samples | Zendy

Iva Martincová | Zendy; Tatiana Aghová | Zendy

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Comparison of 12 DNA extraction kits for vertebrate samples

Author(s) -

Iva Martincová,

Tatiana Aghová

Publication year - 2020

Publication title -

animal biodiversity and conservation

Language(s) - Spanish

Resource type - Journals

SCImago Journal Rank - 0.39

H-Index - 34

eISSN - 2014-928X

pISSN - 1578-665X

DOI - 10.32800/abc.2020.43.0067

Subject(s) - dna extraction , extraction (chemistry) , dna , biology , genomic dna , vertebrate , yield (engineering) , protocol (science) , computational biology , sample (material) , microbiology and biotechnology , chromatography , polymerase chain reaction , genetics , chemistry , pathology , medicine , gene , materials science , alternative medicine , metallurgy

espanolObtener extracciones de ADN de buena calidad es un paso crucial para los proyectos de investigacion del ambito de la biologia molecular. En la actualidad, existen numerosos protocolos para la extraccion en tejidos de vertebrados. En el presente estudio comparamos 11 protocolos de extraccion por columnas y un protocolo HotSHOT utilizando condiciones parecidas (como el tipo de muestra o el peso del material inicial). Evaluamos el tiempo de extraccion, la calidad y la cantidad de ADN obtenido y el precio de la extraccion de una unica muestra. Segun nuestro analisis, los kits que dieron mejores resultados para producir ADN con la mayor concentracion y pureza son JetQuick® Genomic DNA Purification Kit (Genomed) y el NucleoSpin® Tissue (Macherey–Nagel). No obstante, se recomienda encarecidamente probar varios kits de extraccion con muestras especificas para encontrar el que sea mejor en cuanto al tiempo, la calidad y el costo en relacion con un proyecto concreto. EnglishObtaining high quality DNA extractions is a crucial step for molecular biology research projects. At present, numerous protocols are available for vertebrate tissue extractions. In the present study we compared eleven column–based protocols and one HotSHOT protocol using similar conditions (i.e., type of sample, weight of starting material). We evaluated time of extraction, quality and quantity of DNA yield, and price of extraction for a single sample. Based on our analysis, the most successful kits for producing DNA with the highest concentration and purity are the JetQuick® Genomic DNA Purification Kit (Genomed) and the NucleoSpin® Tissue (Macherey–Nagel). Nevertheless, it is highly recom-mended to test various extraction kits with specific samples to find the optimal kit in all aspects of time, quality and cost for a particular project

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