Immune strategies of phagoctytic cells stimulated in vitro with live and heat-inactivated Streptococcus pyogenes | Zendy

Agust韓 C閟ar M諬LINGERa | Zendy; Virginia Paula SIFFREDIa | Zendy; Serenela CHULIBERTa | Zendy; Adriana PONESSAb | Zendy; Rodolfo David NOTARIOb | Zendy; Diana Graciela DLUGOVITZKYa | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Immune strategies of phagoctytic cells stimulated in vitro with live and heat-inactivated Streptococcus pyogenes

Author(s) -

Agust韓 C閟ar M諬LINGERa,

Virginia Paula SIFFREDIa,

Serenela CHULIBERTa,

Adriana PONESSAb,

Rodolfo David NOTARIOb,

Diana Graciela DLUGOVITZKYa

Publication year - 2013

Publication title -

biocell

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.182

H-Index - 27

eISSN - 1667-5746

pISSN - 0327-9545

DOI - 10.32604/biocell.2013.37.063

Subject(s) - streptococcus pyogenes , microbiology and biotechnology , peripheral blood mononuclear cell , respiratory burst , streptococcus pneumoniae , flow cytometry , streptococcus , biology , immune system , in vitro , immunology , bacteria , biochemistry , antibiotics , staphylococcus aureus , genetics

Streptococcus pyogenes (group A Streptococcus) is frequently involved in a wide range of human diseases. Here we evaluated polymorphonuclear neutrophils and mononuclear cells from healthy subjects for their bactericidal function after stimulation with live and inactivated Streptococcus pyogenes (Streptococcus Group A). Mononuclear cells and Neutrophils were isolated from heparinized blood samples (n=18) using a Ficoll-Hypaque gradient and cultured in RPMI 1640 for 18 hours with a suspension of either live or inactivated Streptococcus pyogenes. Both the respiratory burst (flow cytometry) and nitrite, TNF and IL17 production (ELISA) were measured in the cell culture supernatants. An increased respiratory burst (expressed as R index) was induced by both live and inactivated bacteria. Also, increased nitrite, TNF and IL17 concentrations were found in cell culture supernatants in both cases. These findings may provide some explanation as to the roles played by neutrophils and mononuclear cells in Streptococcus pyogenes immunopathogenicity.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research