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Perennial warm-seasongrasses including switchgrass (Panicum virgatum L.), big bluestem(Andropogon geradii Vitman), and Indiangrass (Sorghastrum nutans L.) havedrawn interest as bioenergy feedstocks due to their high yielding capacitywith minimal amounts of inputs under a wide range of environments, and theircapability to produce multiple environmental benefits. Nitrogen (N)fertility and harvest timing are considered as critical management practiceswhen optimizing biomass yield and the feedstock quality of these grasses.The objective of this investigation was to quantify the impact of N fertilizer rate, N timing and harvest date on warm season biomass dry matter yield. Research was conducted in 2014 and 2015 on a total of four field-plot locations situated in central and west-central Missouri. Nitrogen fertilizer was applied using dry ammonium nitrate at the rates of 0, 34, 67, and 101 kg ha-1 at two application times, all N early spring and split N (early spring and following 1st harvest). Harvest treatments were as follows: 1) one cut in September; 2) one cut in November; 3) one cut in June and a second in September; and 4) one cut in June and a second in November. Treatments were arranged in a split-plot design with N rate as the main plot and harvest as the sub-plot in arandomized complete block design. Both N and harvest date and their interactions impacted biomass yield at all four locations. Delaying harvesting until late fall or killing frost increased yield. November harvest in combination with N rates grater than or equal to 67 kg ha-1 year-1 produced higher yields compared to the control and 34 kg ha-1N treatments and other harvest timing strategies. Although N was needed to optimize yield, partial factor 24 productivity (PFP) of applied N was flat when N applied was greater than 34 kg ha-1. Nitrogen fertilization at 67 kg ha-1 per growing season provided an opportunity to maintain a balance between both yield and efficiency of N inputs. Results of this research highlight the interactions of N fertilization and harvest management have when optimizing yield of warm-season grasses grown as bioenergy feedstocks. List of acronyms: N, Nitrogen; PFP, partial factor productivity.	

Nitrogen and harvest impact on warm-season grass biomass yield and feedstock quality