QUANTIFICATION OF ISOORIENTIN IN THREE VARIETIES OF PASSION FRUIT PEEL ETHANOLIC EXTRACT BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY

The utilization of passion fruit peel is still very minimal and has not been used optimally. The phytochemical content contained in the passion fruit peel is very diverse and can provide diverse pharmacological activities. The different varieties of passion fruit peel that are seen from different peel colors, allow causing differences in the phytochemical content and differences in the pharmacological effects in the passion fruit peel. Analysis of isoorientin content contained in passion fruit peel extract with different varieties (purple, red, and yellow) of passion fruit by high-performance liquid chromatography mass spectrometry methods. The passion fruit peel with different varieties was extracted by maceration with ethanol. Analysis of the content of isoorientin was carried out by high performance liquid chromatography mass spectrometry method. Stationary phase octadecylsilane column; Mobile phase used is a gradient system with aqueous formic acid 0.1% (A) and acetonitrile (B). The content of isoorientin in ethanolic extract of purple passion fruit peel was 75.14 mg of isoorientin per kg of dried simplicial. The content of isoorientin in ethanolic extract of red passion fruit peel was 50.16 mg of isoorientin per kg of dried simplicial. The content of isoorientin in ethanolic extract of yellow passion fruit peel was 30.11 mg of isoorientin per kg of dried simplicia. There is a significant difference in the content of isoorientin in ethanolic extract of passion fruit peel with different varieties. The highest isoorientin content is in ethanolic extract of purple passion fruit peel. The lowest isoorientin content is in ethanolic extract of yellow passion fruit peel.