FLAVONE CONTENT ANALYSIS OF WOGONIN (5,7- DIHYDROXY-8-METHOXY) FROM Tetracera indica L. MERR

One of the plants that contain flavonoid compounds is the Tetracera indica L. Merr plant which comes from the Dilleniaceae family and is known by the common name of site. Phytochemical tests on the part of the grading fruit indicate the presence of phenolic compounds, namely flavonoids, so it is necessary to isolate and identify the compounds contained in the grading fruit. Compounds 9, 12-Octadecadienoic acid (Z,Z), Flavone, 5,7-dihydroxy-8methoxy, and Hexadecanoic acid are three compounds with the largest content and are contained in all parts of Tetracera. indica. This experiment was conducted to determine the content and to analyze the content of flavone, 5,7 dihydroxy-8-Methoxy (wogonin) in the Tetracera indica L. Merr plant. This experiment was conducted in October 2018 to March 2019. Analysis of the wogonin compound was carried out using HPLC (Shimadzu LC20AD) equipped with a Luna C18 column. 5 μm (150 x 4.6 mm, Phenomenex), with a wavelength of 270 nm. Wogonin compounds were found in the Tetracera indica plant extract at a retention time of 18.053 minutes from the reference standard and extract using spike injection at a wavelength of 270 nm by the HPLC instrument in this study.