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The Mean Centered of Spectra Ratio (MCSR) method is a practical and straight forward method to the determination of Metformin and Glibenclamide in tablet preparations using mathematical software models. The MCSR method has manipulated an absorption to amplitude and change the wavelength of Metformin from 235.6 nm to 244.4 nm and Glibenclamide from 228.2 nm to 210.3 nm. The method had met the requirements of method validation following ICH 2005. The statistical results showed that the levels were in accordance with the requirements of tablet preparations according to Indonesian Pharmacopoeia Edition V. The MCSR application had met the validation requirements for Metformin and Glibenclamide levels in tablet preparations.

SIMULTANEOUS ASSAY OF METFORMIN AND GLIBENCLAMIDE IN COMBINED DOSAGE FORM BY MEAN CENTERED OF SPECTRA RATIO METHODS