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The stem bark of V. pubescens Vahl has been used as a traditional medicine in Indonesia. Isolation of the stem bark of V. pubescens Vahl with some column chromatography techniques led to obtaining the lactone diterpenoids, neoandrographolide (1), andrographolide (2), 14-deoxyandrographolide (3), and a pentacyclic triterpenoid compound, betulinic acid (4). Neoandrographolide (1) was reported in this plant for the first time. Cytotoxic activity of compounds 1-4 showed inactivity against HeLa (MTT assay).

CYTOTOXIC LACTONE-TYPE DITERPENOIDS AND TRITERPENOID FROM Vitex pubescens Vahl