HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY ANALYSIS FOR α-CYPERONE AND NOOTKATONE FROM THE TUBER OF NUTSEDGE (Cyperus rotundus L.) IN THE TROPICS | Zendy

Ferdhi Isnan Nuryana | Zendy; Muhamad Achmad Chozin | Zendy; Dwi Guntoro | Zendy

Open Access

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY ANALYSIS FOR α-CYPERONE AND NOOTKATONE FROM THE TUBER OF NUTSEDGE (Cyperus rotundus L.) IN THE TROPICS

Author(s) -

Ferdhi Isnan Nuryana,

Muhamad Achmad Chozin,

Dwi Guntoro

Publication year - 2019

Publication title -

rasayan journal of chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.281

H-Index - 22

eISSN - 0976-0083

pISSN - 0974-1496

DOI - 10.31788/rjc.2019.1215024

Subject(s) - cyperus rotundus , cyperus , chemistry , chromatography , biology , horticulture

Two primary compounds from the sesquiterpene group contained in nutsedge’s tuber, α-cyperone and nootkatone are related to its benefits as herbal medicine and natural pesticides. This research was conducted to analyze the content of α-cyperone and nootkatone at three different part of the tuber (whole-tuber, peeled-tuber, and tuber’s peel) so that it indicates the level of activity from these parts of nutsedge tuber in the tropics. The ethanolic extracts were analyzed using high-performance liquid chromatography (HPLC) equipped with C18 column, acetonitrile and aquabidestillata (65:35) as a mobile phase and PDA detection at 254 nm. The analysis showed the results that the highest content of α-cyperone was on the whole-tuber 1.074%, followed by peeled-tuber 0.736% and tuber’s peel 0.202%. Nootkatone was not found in this nutsedge’s tuber.

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