DETERMINATION OF ANTIOXIDANT ACTIVITY, TOTAL PHENOLIC CONTENT, AND TOTAL FLAVONOID CONTENT OF ROOTS, STEM BARK, AND LEAVES OF Elaeocarpus mastersii KING

In this research, antioxidant activity, total phenolic, and flavonoid content of n-hexane, ethyl acetate, and methanol fractions of roots, stem bark, and leaves of Elaeocarpus mastersii King were determined by the colorimetric method using DPPH assay (1,1-diphenyl-2-picrylhydrazyl), Folin-Ciocalteu reagent, and aluminum chloride reagent, respectively. Phytochemical screening was evaluated by specific reaction of constituents to the specific reagent. Ethyl acetate fraction had the best value of phenolic content and antioxidant activity among all fractions which the best value was obtained from the leaves (380.99 ± 2.14 mg GAE/g DW and IC50 1.95 ± 0.01 μg/mL) followed by the roots (362.88 ± 1.89 mg GAE/g DW and IC50 2.05 ± 0.01 μg/mL) and the stem bark (341.89 ± 3.97 mg GAE/g DW and IC50 2.36 ± 0.02 μg/mL). All fractions of this plant showed the low value of flavonoid content. The phytochemical screening exhibited that this plant was dominated by phenolic and alkaloid compounds. These results demonstrate the great potential of Elaeocarpus mastersii King as a natural antioxidant and active compounds.