ANALYSIS OF COMPOUNDS AND IMMUNOSTIMULATORY PROPERTIES OF CURCUMA MANGGA RHIZOMES ON MALE MICE

The current study was carried out to determine the constituents in ethanol extract of Curcuma mangga rhizomes and its immunostimulatory effects of the extract on cellular and humoral immunity of male mice. Determination of constituents in the extract was conducted using Ultra Performance Liquid Chromatography. The ethanol extract of Curcuma mangga was comprised to delayed-type hypersensitivity response and haemagglutinating antibody titre tests for its immunostimulatory potential. The test animal received extract at doses of 100, 200 and 400 mg/kg BW for 7 days after sensitized by bovine Red Blood Cells. Cellular response was measured by increase of paw volume, meanwhile humoral antibody response was determined by hemagglutination method. Quercetin was determined quantitatively in the extract by a validated reversed phase Ultra Performance Liquid Chromatography method. The footpad thickness and antibody titre of male mice after treatment with ethanol extract of Curcuma mangga were significantly higher as compared to negative control (P<0.05). The extract of Curcuma mangga at a dose of 400 mg/kg BW depicted stronger immnumostimulatory activity as compared to positive control. The result indicates that the ethanol extract of Curcuma mangga shows immunostimulatory activity, emphasizing its potential to be developed as a therapeutic agent.