DETERMINATION OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, AND ANTIMUTAGENIC ACTIVITY OF ETHANOL EXTRACT NANOPARTICLES OF RHAPHIDOPHORA PINNATA (L.F) SCHOTT LEAVES

Phenolic and flavonoid compounds in plants are a natural source of antioxidants which can inhibit the development of several types of diseases in humans, such as cardiovascular diseases and cancer. This study aimed to determine total phenolic content, total flavonoid content, and antimutagenic activity of ethanol extract nanoparticles of Rhaphidophora pinnata (L.f) Schott leaves. The total phenolic content and the total flavonoid content of Rhaphidophora pinnata extract was determined with Folin-Ciocalteu method and aluminum chloride. The antimutagenic activity of the ethanol extract of Rhaphidophora pinnata leaves was evaluated based on the bone marrow micronucleus assay method. The total phenolic content of the ethanol extract of Rhaphidophora pinnata was 49.2 mg gallic acid equivalent/g and the total flavonoid content was 21.8 mg quercetin equivalent/g. The administration of ethanol extract nanoparticles of Rhaphidophora pinnata at a dose of 200, 400, 800 mg/kgBW could decrease the formation of micronucleus in the spinal cord of rats compared to the cyclophosphamide group (P<0.05). The ethanol extract nanoparticles of Rhaphidophora pinnata had antimutagenic activity and dosedependent protection effects against cyclophosphamide – inducer of DNA oxidative damage.