Measurement of Retinoids and .BETA.-Carotene 15, 15'-Dioxygenase Activity in HR-1 Hairless Mouse Skin with UV Exposure | Zendy

Atsuko Takeda | Zendy; Takao Morinobu | Zendy; Kimitaka Takitani | Zendy; Mieko Kimura | Zendy; Hiroshi Tamai | Zendy

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Measurement of Retinoids and .BETA.-Carotene 15, 15'-Dioxygenase Activity in HR-1 Hairless Mouse Skin with UV Exposure

Author(s) -

Atsuko Takeda,

Takao Morinobu,

Kimitaka Takitani,

Mieko Kimura,

Hiroshi Tamai

Publication year - 2003

Publication title -

journal of nutritional science and vitaminology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.41

H-Index - 56

eISSN - 1881-7742

pISSN - 0301-4800

DOI - 10.3177/jnsv.49.69

Subject(s) - hairless , retinoid , chemistry , vitamin , beta carotene , retinol , dioxygenase , retinyl acetate , metabolism , alpha (finance) , medicine , endocrinology , biochemistry , biology , retinoic acid , enzyme , gene , construct validity , nursing , patient satisfaction

We investigated the vitamin A status and beta-carotene 15,15'-dioxygenase activity in hairless mice with UV exposure to assess the regulation of vitamin A metabolism after UV irradiation. HR-1 hairless mice were irradiated with UV at 3 J/cm2 for 5 d. After UV irradiation, the mice were sacrificed and samples were obtained to analyze the retinoid concentration, expression of RXR-alpha, and beta-carotene 15,15'-dioxygenase activity. After UV exposure, the skin retinoid concentration was significantly lower as well as the expression of RXR-alpha. Higher skin beta-carotene dioxygenase activity was observed in the UV group as compared to the control group. We found no significant differences in the alpha-tocopherol concentration or acrolein levels in the skins of the two groups. In conclusion, the elevation of beta-carotene 15,15'-dioxygenase activity in hairless mice after UV exposure may be a response to reduction of the skin retinoid concentration.

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