Open AccessMeasurement of Retinoids and .BETA.-Carotene 15, 15'-Dioxygenase Activity in HR-1 Hairless Mouse Skin with UV Exposure
Author(s) -
Atsuko Takeda,
Takao Morinobu,
Kimitaka Takitani,
Masao Kimura,
Hiroshi Tamai
Publication year - 2003
Publication title -
journal of nutritional science and vitaminology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.41
H-Index - 56
eISSN - 1881-7742
pISSN - 0301-4800
DOI - 10.3177/jnsv.49.69
Subject(s) - hairless , retinoid , chemistry , vitamin , beta carotene , retinol , dioxygenase , retinyl acetate , metabolism , alpha (finance) , medicine , endocrinology , biochemistry , biology , retinoic acid , enzyme , gene , construct validity , nursing , patient satisfaction
We investigated the vitamin A status and beta-carotene 15,15'-dioxygenase activity in hairless mice with UV exposure to assess the regulation of vitamin A metabolism after UV irradiation. HR-1 hairless mice were irradiated with UV at 3 J/cm2 for 5 d. After UV irradiation, the mice were sacrificed and samples were obtained to analyze the retinoid concentration, expression of RXR-alpha, and beta-carotene 15,15'-dioxygenase activity. After UV exposure, the skin retinoid concentration was significantly lower as well as the expression of RXR-alpha. Higher skin beta-carotene dioxygenase activity was observed in the UV group as compared to the control group. We found no significant differences in the alpha-tocopherol concentration or acrolein levels in the skins of the two groups. In conclusion, the elevation of beta-carotene 15,15'-dioxygenase activity in hairless mice after UV exposure may be a response to reduction of the skin retinoid concentration.