Open AccessTreatment of traumatic brain injury in mice with bone marrow stromal cell–impregnated collagen scaffolds
Author(s) -
Changsheng Qu,
Ye Xiong,
Asim Mahmood,
David L. Kaplan,
Anton Goussev,
Ruizhuo Ning,
Michael Chopp
Publication year - 2009
Publication title -
journal of neurosurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.564
H-Index - 210
eISSN - 1933-0693
pISSN - 0022-3085
DOI - 10.3171/2009.4.jns081681
Subject(s) - medicine , lesion , stromal cell , traumatic brain injury , pathology , bone marrow , transplantation , saline , surgery , anesthesia , psychiatry
Object This study was designed to investigate new ways of delivering human marrow stromal cells (hMSCs) to the injured brain by impregnating them into collagen scaffolds in a mouse model of traumatic brain injury (TBI). Methods Eight C57BL/6 J mice were injured with controlled cortical impact and received transplantation into the lesion cavity of 0.3 × 10 6 hMSCs impregnated into 3D porous collagen scaffolds. Additional experimental groups of 8 mice each received scaffolds implanted alone into the lesion cavity, hMSCs administered alone intracerebrally or intravenously, or saline injected into the lesion core. All treatments were performed 7 days after TBI. Spatial learning was measured using a modified Morris water maze test, and brain tissue samples were processed for histopathological analysis. Results The results showed that hMSC-impregnated scaffolds were more effective than hMSCs administered alone (either intravenously or intracerebrally) in improving spatial learning, reducing lesion volume, and increasing vascular density after TBI. Conclusions Collagen scaffolds populated with hMSCs may be a new way to reconstruct injured brain tissue and improve neurological function after TBI.