Neutralizing Monoclonal Antibodies to Striped Jack Nervous Necrosis Virus (SJNNV). | Zendy

Toyohiko Nishizawa | Zendy; Masaaki Kise | Zendy; Toshihiro Nakai | Zendy; Kiyokuni Muroga | Zendy

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Neutralizing Monoclonal Antibodies to Striped Jack Nervous Necrosis Virus (SJNNV).

Author(s) -

Toyohiko Nishizawa,

Masaaki Kise,

Toshihiro Nakai,

Kiyokuni Muroga

Publication year - 1995

Publication title -

fish pathology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.24

H-Index - 43

eISSN - 1881-7335

pISSN - 0388-788X

DOI - 10.3147/jsfp.30.111

Subject(s) - monoclonal antibody , virology , biology , virus , antibody , microbiology and biotechnology , immunology

Viral nervous necrosis (VNN) caused by a nodavirus (striped jack nervous necrosis virus : SJNNV) is a serious problem in seed production process of striped jack Pseudocaranx dentex in Japan. Mouse monoclonal antibodies (MAbs) to SJNNV were produced by using a homogenate of infected larval striped jack. Among 8 MAbs reacting with the homogenate of infected larvae in enzyme-linked immunosorbent assay (ELISA), three MAbs (SJ-102B, SJ-204D, SJ-207C) were proved to recognize the 42 kDa coat protein of SJNNV by Western blot analysis. An in vivo neutralization test, where larval striped jack were exposed to purified SJNNV (100ng/ml) or the virus treated with MAbs (1mg) and the resultant infection with SJNNV in larvae was monitored by ELISA using an anti-SJNNV rabbit serum, exhibited that two MAbs SJ-102B and SJ-204D have neutralizing activity against SJNNV.

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