FABRICATION AND CHARACTERIZATION OF PHOTOPATTERNED POLYMER MEMBRANES FOR PROTEIN CONCENTRATION AND DIALYSIS IN MICROCHIPS | Zendy

Simon Song | Zendy; Anup K. Singh | Zendy; Timothy J. Shepodd | Zendy; Brian J. Kirby | Zendy

Open Access

FABRICATION AND CHARACTERIZATION OF PHOTOPATTERNED POLYMER MEMBRANES FOR PROTEIN CONCENTRATION AND DIALYSIS IN MICROCHIPS

Author(s) -

Simon Song,

Anup K. Singh,

Timothy J. Shepodd,

Brian J. Kirby

Publication year - 2004

Publication title -

1998 solid-state, actuators, and microsystems workshop technical digest

Language(s) - English

Resource type - Conference proceedings

DOI - 10.31438/trf.hh2004.107

Subject(s) - membrane , fabrication , polymer , materials science , dialysis , nanoporous , porosity , analyte , synthetic membrane , characterization (materials science) , dialysis tubing , chemical engineering , chromatography , nanotechnology , chemistry , composite material , engineering , medicine , biochemistry , alternative medicine , pathology

Unique capability for processing protein samples in microchips is demonstrated via fabrication of photopatterned nanoporous polymer membranes with engineered pore size. Proteins are electrophoresed into membranes and concentrated by over two orders of magnitude before being injected for analysis. Specific analytes can be extracted from complex samples by flowing sample past a long dialysis membranes and perfusing in counterflow. Protein processing performance is used to characterize membrane porosity and specific diffusivities.

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