Discrepancies in the diagnosis of methicillin resistant Staphylococcus aureus among different hospitals in Lahore, Pakistan | Zendy

T Romeeza | Zendy; Ahmed Nadeem | Zendy; Javaid Nauman | Zendy; A. Khalifa Afia | Zendy; R Kunwal | Zendy

Open Access

Discrepancies in the diagnosis of methicillin resistant Staphylococcus aureus among different hospitals in Lahore, Pakistan

Author(s) -

T Romeeza,

Ahmed Nadeem,

Javaid Nauman,

A. Khalifa Afia,

R Kunwal

Publication year - 2009

Publication title -

iium medical journal malaysia

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.139

H-Index - 9

eISSN - 2735-2285

pISSN - 1823-4631

DOI - 10.31436/imjm.v8i2.750

Subject(s) - medicine , staphylococcus aureus , latex fixation test , coagulase , microbiology and biotechnology , antibiotic sensitivity , antibiotics , antibiotic resistance , agar diffusion test , serology , staphylococcus , bacteria , immunology , biology , genetics , antibody

Staphylococcus aureus is a pathogen causing a wide range of infections. It changes its genome under the selection pressure of antibiotics. Methicillin resistance is commonly determined by Kirby-Bauer disc diffusion method which has low sensitivity and specificity. Thus, anti PBP-2a latex agglutination test was used to confirm the identity of those labeled as MRSA. Materials and Methods: One hundred bacterial samples characterized as MRSA according to Kirby-Bauer diffusion method were collected from different hospitals in Lahore. Results: Biochemical characterization of these MRSA isolates revealed that only 64 (64%) were actually S. aureus while the other 36 isolates (36%) were coagulase negative staphylococci. Further serological characterization revealed that among the 64 isolates of S. aureus, 50 (78%) were MRSA while 14 (22%) were MSSA. Conclusion: Therefore, overall, 50% of the bacterial strains were misidentified as MRSA. Misdiagnosis of antibiotic resistance may lead to an inappropriate prescription of medicines to these patients. KEY WORDS: MRSA, MSSA, Staphylococcus epidermidis, Agglutination

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