In vitro effect of different cytokinin types (BAP, TDZ) on two different Ocimum basilicum cultivars explants | Zendy

E. Enkhbileg | Zendy; Miklós Fári | Zendy; Erika Kurucz | Zendy

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In vitro effect of different cytokinin types (BAP, TDZ) on two different Ocimum basilicum cultivars explants

Author(s) -

E. Enkhbileg,

Miklós Fári,

Erika Kurucz

Publication year - 2019

Publication title -

international journal of horticultural science

Language(s) - English

Resource type - Journals

eISSN - 2676-931X

pISSN - 1585-0404

DOI - 10.31421/ijhs/25/3-4/3930

Subject(s) - basilicum , ocimum , explant culture , cytokinin , sweet basil , callus , shoot , biology , botany , horticulture , cultivar , herb , in vitro , auxin , traditional medicine , medicinal herbs , gene , medicine , biochemistry

Ocimum basilicum L. (sweet basil) is an economically and ethnobotanically important aromatic, medicinal, ornamental and culinary herb, with a very wide gene pool, that is sensitive to cold and prone to several plant pathogens that can demolish harvest and lessen yield. In this research, the effects of BAP (6-Benzylaminopurine) and TDZ (Thidiazuron) on different genotypes for in vitro cloning were determined, in order to provide a detailed protocol guide concerning Ocimum basilicum L. propagation. The results from the O. basilicum seed propagations revealed that the best condition for the secondary shoot growth is with 5.0 mg/l TDZ or 1.5 mg/l BAP on all types of explants except the root, the secondary root growth can be obtained on all types explant with any BAP concentration and all cytokinins can induce callus on all types of explants. On the whole, it shows that multiple secondary shoot induction and regeneration in Ocimum basilicum L. is regulated by appropriate cytokinin concentration.

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