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Ceftriaxone is a third generation antibiotic from the class of cephalosporin, which is acted as antibiotic specifically for pneumonia and urinary tract infections. This study aim was to validate the HPLC method for analysing raw ceftriaxone. System suitability, selectivity, linearity, accuracy, precision, LOD and LOQ were applied as the analysis parameters. Analysis was carried out using Inertsil ODS column 3, C-18, (5mm, 4.6 x 150 mm) withmobile phase comprising methanol and 0.1% water TFA in a ratio of 35:65 (v / v) at a flow rate of 1.0 mL / minute. The column temperature was conditioned at 250C and the analysis was performed using a PDA detector (photodiode array) at 235 nm. This study showed excellent linear response with correlation coeffient (R2) values of with RSD 0,31%. In addition this method revealed that the limit of detection was at 1.41 μg/mL and limit of quantification was achieved at 4.69 μg/mL Kata kunci: ceftriaxone, validation, HPLC

Pengembangan dan Validasi Metode High Performance Chromatography (HPLC) untuk Analisis Sodium Seftriakson