Open AccessA New Series of S-Adenosyl-L-Methionine Synthetase Inhibitors
Author(s) -
Karine Lavrador,
Brigitte Allart,
D. Guillerm,
Georges Guillerm
Publication year - 1998
Publication title -
journal of enzyme inhibition
Language(s) - English
Resource type - Journals
eISSN - 1029-2462
pISSN - 1026-5457
DOI - 10.3109/14756369809021481
Subject(s) - methionine , methionine adenosyltransferase , enzyme , biochemistry , amino acid , recombinant dna , gene isoform , enzyme inhibitor , chemistry , stereochemistry , biology , gene
A new series of epithio and epoxy amino acid analogues of L-methionine or L-methoxinine were examined as potential inhibitors of the enzyme S-adenosylmethionine (AdoMet) synthetase. The kinetic behaviour of these compounds was studied using recombinant rat liver S-adenosyl-L-methionine sythetase (alpha-isoform) fractionated from E. coli, transformed with the plasmid pSSRL-T7N. All the compounds tested were competitive inhibitors with respect to L-methionine and the (2S, 4S)-2-amino-4,5-epoxy pentanoic acid was found to be a very potent inhibitor of the enzyme compared to those already reported for AdoMet synthetase from other mammalian tissues.