Open AccessExperiments with Immunization of Mice with Blastocysts by an Intrasplenic Route
Author(s) -
Bo Nilsson,
Kjell-Olov Grönvik,
Peter Svalander
Publication year - 1983
Publication title -
upsala journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.808
H-Index - 41
eISSN - 2000-1967
pISSN - 0300-9734
DOI - 10.3109/03009738309178448
Subject(s) - antibody , medicine , spleen , antigen , antiserum , blastocyst , andrology , immunization , immunology , immune system , microbiology and biotechnology , embryo , biology , embryogenesis
A few attaching blastocysts from CBA/H mice were irradiated from a Cesium source and transferred into the spleen of male DBA2 mice. A booster immunization was performed after four weeks. Blood samples for preparation of antiserum to test for the presence of immunoglobulins directed against blastocyst surface determinants were obtained by a retro-orbital puncture. Specific antibodies were detected with a protein A-gold method, modified for transmission electron microscopy of air-dried blastocysts. The results showed that CBA/H blastocyst incubated in DBA2 immune serum were positive for protein A-gold labelling, while control blastocysts only possessed a few irregularly scattered gold particles. Thus, it seems as a deposition of antigens in the spleen tissue with persistence of the antigens at this site will result in detectable antibodies in the peripheral blood.