Formulation, Evaluation and HPTLC Analysis of Topical Ointment Containing Calendula officinalis and Echinacae purpurae Mother Tinctures

Skin morbidities are the major threat faced by the young adults of India. Various factors like climate, genetic factors, environmental factors, hygiene, dietary habits etc. Contribute to this ailments. There are both oral and topical medication for treating skin diseases. Antimicrobial agents in creams, ointments, lotions etc. Are used for the dermal treatments when bacterial and fungal attack is suspected or diagnosed. In this study, an ointment for treating skin morbidities was formulated using calendula officinalis and echinacae purpurae mother tinctures,  evaluation and HPTLC profiling of the formulated ointment was performed with toluene: ethyl acetate: formic acid: methanol in the ratio 14:10:2:1 as mobile phase with standard procedures and scanned with  camag TLC scanner 3 at 254 and 366nm.the HPTLC profiling of the simple ointment and mother tincture ointments showed 8 spots and 7 spots at 254 nm respectively.