Open AccessMolecular marker assisted detection of the mycoparasite <i>Coniothyrium minitans</i> A69 in soil
Author(s) -
Hayley J. Ridgway,
Alison Stewart
Publication year - 2000
Publication title -
proceedings of the new zealand weed and pest control conference/new zealand plant protection/proceedings of the ... national weeds conference/proceedings of the new zealand weed control conference/proceedings of the new zealand plant protection conference
Language(s) - English
Resource type - Journals
eISSN - 0370-2804
pISSN - 0370-0968
DOI - 10.30843/nzpp.2000.53.3661
Subject(s) - biology , sclerotinia sclerotiorum , spore , biological pest control , fungus , sclerotinia , spore germination , germination , pathogen , horticulture , polymerase chain reaction , inoculation , botany , microbiology and biotechnology , gene , biochemistry
Coniothyrium minitans A69 has been shown to have biological control activity against the plant pathogen Sclerotinia sclerotiorum and a PCR based assay has been developed to specifically identify this isolate The practical application of this PCR assay for detection of C minitans from soil was assessed Sterile and nonsterile soil was inoculated with spores from C minitans A69 at five different concentrations and DNA recovered using a SDS/Phenol/Chloroform method A number of factors affected DNA recovery and subsequent PCR with a maximum sensitivity of down to 1x102 spores/g soil achieved in sterile soil Detection of C minitans in nonsterile soil was hampered by failure of the fungus to germinate However this method has improved throughput and cost effectiveness compared with conventional detection methods involving quantitative colony recovery