Biofilm Formation and β-lactamase Enzymes: A Synergism Activity in Acinetobacter baumannii Isolated from Wound Infection
Author(s) -
Mahyar Porbaran,
Reza Habibipour
Publication year - 2019
Publication title -
journal of advances in medical and biomedical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.113
H-Index - 12
ISSN - 2676-6264
DOI - 10.30699/jambs.27.125.34
Subject(s) - biofilm , acinetobacter baumannii , microbiology and biotechnology , enzyme , acinetobacter , wound infection , chemistry , neisseriaceae , biology , bacteria , antibiotics , medicine , biochemistry , pseudomonas aeruginosa , surgery , genetics
Materials & Methods: Eightynine A. baumannii isolates were collected from wound specimens and were confirmed by different biochemical tests. The biofilm-producing strains were identified using the crystal violet method. The producing strains of KPC, ESBL, and AmpC β-lactamase enzymes were detected through phenotypic tests. Further, the PCR method was employed to identify the ESBL, KPC, and AmpC. The Chi-square test and SPSS 16 were used for data analysis.
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