Inhibitory Effect of Fluconazole Combined with Amphotericin B on Fluconazole-Resistant Candida albicans Biofilm Formation | Zendy

Sara Neamati | Zendy; Alireza Khodavandi | Zendy; Fahimeh Alizadeh | Zendy

Open Access

Inhibitory Effect of Fluconazole Combined with Amphotericin B on Fluconazole-Resistant Candida albicans Biofilm Formation

Author(s) -

Sara Neamati,

Alireza Khodavandi,

Fahimeh Alizadeh

Publication year - 2019

Publication title -

majallah-i ̒ilmī-i dānishgāh-i ̒ulūm-i pizishkī va khadamāt-i bihdāshtī darmānī-i zanjān

Language(s) - English

Resource type - Journals

eISSN - 2322-4231

pISSN - 1606-9366

DOI - 10.30699/jambs.27.122.1

Subject(s) - fluconazole , biofilm , candida albicans , amphotericin b , microbiology and biotechnology , chemistry , minimum inhibitory concentration , antifungal , corpus albicans , pharmacology , medicine , biology , antibiotics , bacteria , genetics

Materials & Methods: Fluconazole-resistant C. albicans was identified among colonized clinical isolates of C. albicans obtained from the patients at Shahid Beheshti Hospital, Yasooj, Iran, between September 2016 and January 2017. The MICs of fluconazole alone and in combination with amphotericin B were determined on fluconazole-resistant C. albicans by broth microdilution method based on the CLSI document M27-A3 and the synergistic effects were interpreted by the FIC index. To address the inhibitory effects of drug combinations, we investigated the anti-biofilm activities of fluconazole alone and in combination with amphotericin B against fluconazole-resistant C. albicans using crystal violet staining, light field microscopy and expression analysis of HWP1 using RT-PCR.

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