Protective Effect of Egg Yolk Immunoglobulin (IgY) Against Aflatoxin on Blood Parameters, Ileum Morphometry and Hepatocytes’ Histopathology of Broiler Chickens Fed Aflatoxin B1 | Zendy

Ali Nakhaei | Zendy; Nazar Afzali | Zendy; Seyyed Javad Hosseini Vashan | Zendy; Mohammad Amir Karimi Torshizi | Zendy

Open Access

Protective Effect of Egg Yolk Immunoglobulin (IgY) Against Aflatoxin on Blood Parameters, Ileum Morphometry and Hepatocytes’ Histopathology of Broiler Chickens Fed Aflatoxin B1

Author(s) -

Ali Nakhaei,

Nazar Afzali,

Seyyed Javad Hosseini Vashan,

Mohammad Amir Karimi Torshizi

Publication year - 2018

Publication title -

iranian journal of medical microbiology

Language(s) - English

Resource type - Journals

eISSN - 2345-4342

pISSN - 1735-8612

DOI - 10.30699/ijmm.12.3.199

Subject(s) - aflatoxin , broiler , yolk , histopathology , ileum , antibody , clostridium perfringens , biology , immunology , food science , medicine , veterinary medicine , pathology , endocrinology , genetics , bacteria

Materials and Methods: The experiment was based on a completely randomized design with 6 treatments, 4 replications, and 8 observations (chicks). First egg yolks were immunized against aflatoxin by injecting aflatoxin-BSA conjugate to laying hens. Also extracted immunoglobulin with 1 and 0.5 percent /volume concentration was added to broilers' drinking water. The experimental treatments were: 1) control (without any additives); 2) ration contaminated with 1 mg kg aflatoxin B1 (negative control treatment); 3) negative control + 0.5% (V/V) immunized yolk against AFB1, 4) negative control + 0.5% (V/V) unimmunized yolk against AFB1; 5) negative control + 1% (V/V) immunized yolk against AFB1 and 6) negative control + 1% (V/V) unimmunized yolk against AFB1.

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