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A multisyringe liquid chromatographic (MSC) method by using stepwise solvent elution and reverse-phase liquid chromatogra- phy C18 monolithic column to separate Epinephrine (E), Norepineph- rine (NE) and Dopamine (DA) was developed. The first step employs the mixture sodium1-hexanosulfonate monohydrate (HS) 7.5mM: methanol (98:2) to separate NE and E and the second step uses HS (5.0 mM): methanol (92:8) to elute DA. The three analytes were separated on 6 min spending only 10 mL of mobile phases. The detection limits were between 1.3 µg mL -1 (DA) and 2.04 µg mL -1 (NE).

Implementing Stepwise Solvent Elution in Multisyringe Liquid Chromatography (MSC) to Separate Norepinephrine, Epinephrine and Dopamine