Genotype of Cryptosporidiumspp. isolated from human of Al-Qadisiyah province Iraq.

The current study included examination of 100 stool sample from human was collected from AL-Qadisiyah province, from September 2018 until February 2019.the Microscopic examination result showed that oval or spherical shaped with dark pink color or red oocyst on blue ground and 22(22%) positive sample out 100 case . It was recorded that the highest rate 30% (3/10) was seen in September , but the lowest infection rate 13.3% (2/15) was seen in the month of December with no significant differences at level (p<0.05.) regarding to age the high rate of infection 26.31%(10/38)was found in the group less than a year , but the lowest infection rate was observed in the group( >12 years). There is no significant differences at p<0.05.between man and women . In this study the NPCR in molecular examination were used ,the positive sample was13 (59.09%) out of 22 stool sample. Sequencing of a fragment of the (18s rRNA) gene (834 bp) that separated from many different area in ALQadisiyah government recorded (75%)9/12 samplerelated to NCBI – Blast Cryptosporidium hominis isolates ,( 25%) 3/12 sampledisplay closed related to NCBI –Blast Cryptosporidium parvum .In Conclusion The current study concluded that phylogenetic tree and the homology sequences identity gives a clear differentiation of the types of Cryptosporidium parasites which can be isolated at high rates of human in ALDiwaniyah province, which is likely to lead to an outbreak of Cryptosporidiosis in this province.