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A new Schiff base, 2-[(4-Chloro-2-oxo-2H-chromen-3-ylmethylene)-amino]-propionic acid was successfully synthesized and characterized by proton nuclear magnetic resonance (1H NMR), carbon-13 nuclear magnetic resonance (13C NMR), Fourier transform infrared (FT-IR), elemental analysis (CHN) and UV–Vis spectroscopy. It was found out that the synthesized Schiff base could be used as a novel and reliable candidate to detect and quantify of Zn (II) ions in water and biological samples. Some effective parameters on quantification of Zn (II), such as pH, Schiff base and salt concentration were inspected by a (23) full factorial design using Design-Expert, software. Under optimum conditions, the calibration graph was linear in the range 0.04–10.0 μg mL−1 with a limit of detection of 0.01 μg mL−1. The proposed method was successfully applied to the rapid determination of Zn (II) in water and biological samples. The validation was performed by inductively coupled plasma atomic emission spectrometry (ICP-AES) with satisfactory results.

Synthesis a New Schiff Base as a Chelating Agent for Reliable Quantification of Zinc from Water and Biological Samples