Fabrication and Optimization of Linear PEI-Modified Crystal Nanocellulose as an Efficient Non-Viral Vector for In-Vitro Gene Delivery | Zendy

Haghighat Vakilian | Zendy; Eduardo A. Peña Rojas | Zendy; Lida Habibi Rezaei | Zendy; Mehrdad Behmanesh | Zendy

Open Access

Fabrication and Optimization of Linear PEI-Modified Crystal Nanocellulose as an Efficient Non-Viral Vector for In-Vitro Gene Delivery

Author(s) -

Haghighat Vakilian,

Eduardo A. Peña Rojas,

Lida Habibi Rezaei,

Mehrdad Behmanesh

Publication year - 2020

Publication title -

reports of biochemistry and molecular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.467

H-Index - 8

ISSN - 2322-3480

DOI - 10.29252/rbmb.9.3.297

Subject(s) - polyethylenimine , transfection , lipofectamine , gene delivery , viability assay , materials science , cytotoxicity , biophysics , chemistry , nanoparticle , nanocellulose , nanotechnology , microbiology and biotechnology , in vitro , biochemistry , biology , vector (molecular biology) , recombinant dna , gene , cellulose

BackgroundOne of the major challenges in gene therapy is producing gene carriers that possess high transfection efficiency and low cytotoxicity (1). To achieve this purpose, crystal nanocellulose (CNC) -based nanoparticles grafted with polyethylenimine (PEI) have been developed as an alternative to traditional viral vectors to eliminate potential toxicity and immunogenicity.MethodsIn this study, CNC-PEI10kDa (CNCP) nanoparticles were synthetized and their transfection efficiency was evaluated and compared with linear cationic PEI10kDa (PEI) polymer in HEK293T (HEK) cells. Synthetized nanoparticles were characterized with AFM, FTIR, DLS, and gel retardation assays. In-vitro gene delivery efficiency by nano-complexes and their effects on cell viability were determined with fluorescent microscopy and flow cytometry.ResultsPrepared CNC was oxidized with sodium periodate and its surface cationized with linear PEI. The new CNCP nano-complex showed different transfection efficiencies at different nanoparticle/plasmid ratios, which were greater than those of PEI polymer. CNPC and Lipofectamine were similar in their transfection efficiencies and effect on cell viability after transfection.ConclusionCNCP nanoparticles are appropriate candidates for gene delivery. This result highlights CNC as an attractive biomaterial and demonstrates how its different cationized forms may be applied in designing gene delivery systems.

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