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The aim of present experiment was to measure endogenous urinary purine derivatives using feed restriction method, as well as to determine the effect of 4 levels feeding on microbial protein synthesis. The present experiments was conducted by four camels about 326 Kg body weight. The camels were fed by different level of Atriplex leucoclada (AL), including maintenance, 1.25, 1.5 times of maintenance, and ad libitum in Latin square design, and their microbial protein synthesis measure with urinary purine derivatives. In the first experiment, the amount of purine derivatives at fifth day of starvation period, as index for endogenous excretion of urinary purine derivatives determined equal to 14.0 mmol/day. The allantoin was the main urinary purine derivatives excretion in all three starvation period. Urinary excretion of the allantoin decreased with decreasing the level of feeding intake (P<0.05). In the second experiment, urinary excretion of allantoin and total purine derivatives excretion increased with increasing the level of feeding AL (P<0.05) however, urinary excretion of uric acid and xanthine+hypoxanthine not affected with level intake of AL (P<0.05). There was high correlation between digestible organic matter and urinary excretion of purine derivatives. Total absorbed intestinal purine derivatives and flow of microbial N to the duodenum increased with enhancing the level of AL intake (P<0.05). Amount of total urinary purine derivatives excretion was 76.5 g microbial protein per kg digestible OM intake. Therefore, feeding camels with AL in the level of maintenance, the produced metabolizable protein in rumen microbes was not supplied the requirements of camels for metabolizable protein. However, with increasing the level of AL intake higher than maintenance requirement, the camels to metablizable protein not only supplied but also provided favorably.

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Determination the Amount of Microbial Protein Synthesis Using Derivatives of Purines Bases Excreted in the Urine of Dromedary Camels, Fed Different Amounts of Atriplex leucoclada