The Effect of Diclofenac on Caspase-8 and Caspase-9 Activity in Cervical Cancer Cells (HeLa) in Cell Culture | Zendy

Minoo Erfani | Zendy; Masoud Ghorbani | Zendy; Rahim Ahmadi | Zendy

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The Effect of Diclofenac on Caspase-8 and Caspase-9 Activity in Cervical Cancer Cells (HeLa) in Cell Culture

Author(s) -

Minoo Erfani,

Masoud Ghorbani,

Rahim Ahmadi

Publication year - 2018

Publication title -

qom univ med sci j

Language(s) - English

Resource type - Journals

eISSN - 2008-1375

pISSN - 1735-7799

DOI - 10.29252/qums.12.6.1

Subject(s) - hela , apoptosis , cervical cancer , caspase 3 , cell culture , caspase , cell , cervical carcinoma , cancer research , chemistry , cancer , medicine , biology , programmed cell death , biochemistry , genetics

Methods: In this experimental-laboratory study, HeLa cancer cells were purchased from Iran Cell Bank (Pasteur Institute, Tehran, Iran). HeLa cancer cells were cultured in DMEM culture media enriched with 10% FBS, stored in 6 well plate, and incubated for 24 hour. Then, cells were exposed to different concentrations of diclofenac and the viability of cells was evaluated by MTT assay, and the IC50 dose was calculated. The activity of Caspase-8 and caspase-9 on heLa cells, was assayed by ELISA method. The data were analyzed using one-way analysis of variance, Kolmogorov-Smirnov, and Tukey tests.

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