The Effect of Synthesis of Pegylated Nanoliposome Containing DNAi on Reduction of BCL-2 Gene Expression in DLCL2 Cell Line | Zendy

Farahnaz Boozari Saravani | Zendy; Farzaneh Tafvizi | Zendy; Mahmoud Reza Jaafari | Zendy

Open Access

The Effect of Synthesis of Pegylated Nanoliposome Containing DNAi on Reduction of BCL-2 Gene Expression in DLCL2 Cell Line

Author(s) -

Farahnaz Boozari Saravani,

Farzaneh Tafvizi,

Mahmoud Reza Jaafari

Publication year - 2018

Publication title -

qom univ med sci j

Language(s) - English

Resource type - Journals

eISSN - 2008-1375

pISSN - 1735-7799

DOI - 10.29252/qums.12.10.16

Subject(s) - reduction (mathematics) , gene , line (geometry) , cell culture , gene expression , chemistry , cell , microbiology and biotechnology , biology , biochemistry , genetics , mathematics , geometry

Methods: In this study, to prepare DNAi containing pegylated nanoliposome, 3 formulations were prepared with 5, 10, and 15% combinations of PEG 2000 with certain ratios of phospholipid and cholesterols. After purification by a Zetasizer, the mean diameter of nanoliposome was measured. Drug efficacy was determined based on the standard curve of the drug. Cytotoxicity effect of the product was determined by treatment with 0.2, 0.5, and 0.8μg/μl concentrations of each formulation in triplicate in vitro using MTT method. Then, BCL2 gene expression was analyzed in DLCL2 cell line. Data were analyzed using one way ANOVA.

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