Effects of Sub-Minimum Inhibitory Concentrations of Silver Nanoparticles on Some Virulence Factors of Staphylococcus aureus | Zendy

Azizollah Ebrahimi | Zendy; Younes Daryalal | Zendy; Mohammadreza Mahzounieh | Zendy; Sharareh Lotfalian | Zendy

Open Access

Effects of Sub-Minimum Inhibitory Concentrations of Silver Nanoparticles on Some Virulence Factors of Staphylococcus aureus

Author(s) -

Azizollah Ebrahimi,

Younes Daryalal,

Mohammadreza Mahzounieh,

Sharareh Lotfalian

Publication year - 2018

Publication title -

medical laboratory journal

Language(s) - English

Resource type - Journals

ISSN - 2538-4449

DOI - 10.29252/mlj.12.4.7

Subject(s) - staphylococcus aureus , virulence , minimum inhibitory concentration , microbiology and biotechnology , silver nanoparticle , virulence factor , staphylococcus , inhibitory postsynaptic potential , chemistry , nanoparticle , antimicrobial , biology , bacteria , nanotechnology , materials science , biochemistry , gene , genetics , neuroscience

Background and Objectives: Silver nanoparticles (AgNPs) have physical and surface properties that could threaten human and environmental health. AgNPs are classified as ‘very toxic’ to eukaryotic organisms and are less toxic to bacteria. The aim of the present study was to study the effects of different sub-minimum inhibitory concentrations (MICs) of AgNPs on some virulence factors of Staphylococcus aureus as a pathogenic bacterial model. Methods: Tube double serial dilution method was used to determine MIC of AgNPs against standard strain and ten field isolates of S. aureus. Tube cultures of isolates in LB broth were supplemented with different concentrations of AgNPs and were incubated at 37 °C with constant shaking under aerobic conditions. Samples from each tube were streaked on blood agar plates and assay for hemolysins, coagulase and DNase production were

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