Molecular Detection of Hepatitis A Virus in Urban Sewage in Isfahan, Iran | Zendy

Shima Najar | Zendy; Nafiseh Sadat Naghavi | Zendy

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Molecular Detection of Hepatitis A Virus in Urban Sewage in Isfahan, Iran

Author(s) -

Shima Najar,

Nafiseh Sadat Naghavi

Publication year - 2018

Publication title -

medical laboratory journal

Language(s) - English

Resource type - Journals

ISSN - 2538-4449

DOI - 10.29252/mlj.12.4.22

Subject(s) - hepatitis a virus , sewage , virology , medicine , virus , hepatitis virus , geography , environmental science , environmental engineering

Background and Objectives: Hepatitis A virus (HAV) enters sewage through the stool of infected patients and contaminates freshwater and seawater. The aim of present study was quantitative molecular detection of HAV in raw and treated urban sewage samples from Isfahan, center of Iran. Material and methods: Sewage samples were collected from six urban treatment plants in cold and warm weather, between December 2016 and Jun 2017. Physicochemical indicators were measured immediately after sampling. Viral particles were isolated and concentrated by modified adsorption-elution method using Al3+-coated filters. Quantitative PCR was done for amplification of a fragment in the highly conserved 5′ noncoding region of

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