In Vitro Cytotoxicity of Two Categories of Dental Cements | Zendy

Sara Koosha | Zendy; F Nematolahi | Zendy; Maryam Torshabi | Zendy; Fahimeh Tabatabaei | Zendy; Y Borzoo | Zendy; AM Vatandoost | Zendy

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In Vitro Cytotoxicity of Two Categories of Dental Cements

Author(s) -

Sara Koosha,

F Nematolahi,

Maryam Torshabi,

Fahimeh Tabatabaei,

Y Borzoo,

AM Vatandoost

Publication year - 2016

Publication title -

journal of research in dental and maxillofacial sciences

Language(s) - English

Resource type - Journals

eISSN - 2588-4166

pISSN - 2383-2754

DOI - 10.29252/jrdms.1.2.28

Subject(s) - cytotoxicity , dentistry , negative control , cement , distilled water , biocompatibility , positive control , post hoc , dental cement , dentin , chemistry , medicine , in vitro , materials science , composite material , traditional medicine , chromatography , biochemistry , adhesive , organic chemistry , layer (electronics)

ARTICLE INFO Background and Aim: Resin cements are used widely in restorative dentistry regard- less of their biocompatibility. The aim of this study was to compare the cytotoxicity of two categories of dental cements consisting of three chemically set cements (Fuji I, Fuji PLUS and Harvard) and two dual curing cements (BisCem and Duo-Link) by use of MTT assay. Methods and Materials: In this experimental study, four round-shaped samples of each specimen were placed in DMEM culture medium for 24, 48 and 72 hours. The extracts from each sample were applied on L929 mouse fibroblasts. At the end of each period, MTT assay was carried out to estimate the mitochondrial respiration. Data were analyzed by one-way analysis of variance (ANOVA) followed by Tukey's post- hoc test. The degree of cytotoxicity for each sample was determined according to the reference value of the control group. Results:Fuji I cement showed the least cytotoxicity while Harvard and BisCem ce- ments showed the highest cytotoxic effect. The differences were not significant com- pared to the positive control (distilled water). Conclusion: This study showed that dental cements are capable of eliciting biological response in gingival and pulpal cells. They present a potential risk of tissue damage which depends on the cement's brand and curing modes.

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