Evaluation of Phylogenetic Relationships for Waxy Genes in Wild and Cultivated Wheat using Multiplex-PCR | Zendy

Zahra Maryami | Zendy; Arash Fazeli | Zendy; Ali Ashraf Mehrabi | Zendy

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Evaluation of Phylogenetic Relationships for Waxy Genes in Wild and Cultivated Wheat using Multiplex-PCR

Author(s) -

Zahra Maryami,

Arash Fazeli,

Ali Ashraf Mehrabi

Publication year - 2016

Publication title -

journal of crop breeding

Language(s) - English

Resource type - Journals

eISSN - 2676-4628

pISSN - 2228-6128

DOI - 10.29252/jcb.8.18.204

Subject(s) - multiplex polymerase chain reaction , phylogenetic tree , biology , gene , genetics , phylogenetic relationship , multiplex , microbiology and biotechnology , computational biology , polymerase chain reaction

Waxy proteins are responsible for amylase synthesis in wheat seeds, being encoded by three waxy genes (Wx-A1, Wx-B1 and Wx-D1) in hexaploid wheat which have an important role in starch quality. The purpose of this study was to investigate phylogenetic relationship between waxy genes in wild and cultivated wheat using Multiplex-PCR. To this end, 71 populations from 8 Aegilops and 4 wheat wild spices ‘were studied. Clustering results showed that the samples were divided in two main groups. Diploid samples with A, B and D genomes located in the same group but tetra and hexaploid wheat located in the other group. Also, Biplot results showed that Aegilops with A and D genomes had the greatest genetic affinity for waxy genes. Multiplex-PCR technique is able to identify three waxy genes simultaneously in different populations of wheat which contributed substantially to breeder in cost and

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