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Background: Klebsiella species are common causes of nosocomial, ulcers, blood and urinary tract infections, and also acquired pneumonia from the hospital and various intra-abdominal infections. Bacterial resistance mechanisms against antibiotics are different, but one of these resistance mechanisms, which is very problematic, is the production of β-lactamase enzymes in bacteria. The aim of this study was to determine the antibiotic resistance pattern and the presence of beta-lactamase genes in Klebsiella isolated from burn wounds among patients referred to Shahid Motahhari Hospital in Tehran. Materials and methods: 100 strains collected for confirmation of production of broad-spectrum betalactamases (ESBLs) were tested by CDT (Combined Disk Test). Finally, β-lactamase genes were investigated using polymerase chain reaction. Results: The highest resistance rate was observed to ampicillin (93%). 23% of isolates produced ESBLs. The highest frequency of genes was bla shv gene (26.8%). Conclusion: The presence of beta-lactamase genes with high antibiotic resistance is very worrying. Since the present genes can spread through mobile genetic elements in bacteria, among bacteria, it is considered to be a serious alert in the treatment of infections caused by Klebsiella.

majallah-i ̒ulum-i pizishkī./faṣlnāmah-i ̒ulūm-i pizishkī

Evaluation of the prevalence of broad-spectrum beta-lactamases (ESBLs) and carbapenemase genes in Klebsiella pneumoniae strains isolated from burn wounds in patients referred to Shahid Motahari Hospital in Tehran