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Chili sauce sachet A, B and C containing synthetic red coloring agent were taken from three fast food premises in Padang. Synthetic red coloring agent were a food additives used by food manufacturers to enhance color of food products. The use of food coloring agent in Indonesia were regulated in The Rule of Head of the Supervisory Food Board of the Republic of Indonesia Number 37 Year 2013 about the Limit Use of Food Coloring Agent. Thin-layer chromatography (TLC) -densitometry was a precision method for the analysis of food additives. Development and validation of TLC method for chromatographic separation used GF254 silica plate eluted with a mixture of ethanol: butanol: distilled water (4: 5: 5) and spots were detected visually. A red spots on the sample B was identified as Ponceau 4R with Rf value of 0.76. It was then followed by quantitative analysis using densitometry. The linearity of the method was found in the range of 2-10μg/ml with a correlation coefficient of 0.994. Precision intra-day and inter-day relative standard deviation was shown from 1.11% and 2.69% respectively. Accuracy of the method was shown on the percentage of recovery of the 3 different concentrations of solution which gave the average percentage of 108.17%. Limit of detection and limit quantitation was 0.8306μg/ml and 2.7687μg/ml. The concentration of Ponceau 4R in the sample B was 11.9520mg/kg of material. This value was not exceeded the maximum concentration of food coloring agent as stated in the rule which should not be above 70mg/kg of material. ABSTRAK: Saus sambel sachet A, B dan C yang mengandung pewarna merah sintetik diambil ditiga tempat makanan cepat saji dikota padang. Pewarna merah sintetik merupakan salah satu bahan tambahan pangan yang digunakan oleh produsen pangan untuk memberikan sensasi warna pada produk pangannya. Penggunaan pewarna pangan ini diatur dalam Peraturan Kepala Badan Pengawas Makanan Republik Indonesia Nomor 37 Tahun 2013 Tentang Batas Maksimum Penggunaan Bahan Tambahan Pangan Pewarna. Metoda yang presisi untuk analisis bahan tambahan pangan ini adalah kromatografi lapis tipis (KLT)-densitometri. Pengembangan dan validasi metoda KLT untuk pemisahan secara kromatografi digunakan pelat silica GF254 dengan fasa gerak campuran etanol:butanol:aquadest (4:5:5) dan bercak yang nampak dideteksi secara visual. Sebuah bercak merah pada sampel B teridentifikasi mengandung ponceau 4R dengan nilai Rf 0,76 dan dilanjutkan dengan analisis kadar dengan densitometry. Linieritas metode yang dilakukan ditemukan pada rentang 2-10 μg/ml dengan koefisien korelasi 0,994. Presisi intra-day ditunjukkan dari standar deviasi relative 1,11% dan interday 2,69%. Akurasi metode ditunjukkan dari persentase perolehan kembali terhadap 3 konsentrasi yang berbeda dengan persentase rata-rata 108,17%. Batas deteksi dan batas kuatitasi yang didapatkan adalah 0,8306μg/ml dan 2,7687μg/ml. Kadar ponceau 4R yang dikandung dalam sampel B adalah 11,9520 mg/kg bahan yang tidak melebihi batas maksimum penggunaan bahan pewarna menurut peraturan di atas yakni 70mg/ kg bahan.

jsfk (jurnal sains farmasi klinis)/jsfk (jurnal sains farmasi dan klinis)

Development and Validation Of Thin Layer Chromatograpy-Densitometry Method for Determinationa and Quantification of Sinthetic Red Coloring Agent in Sauce Sambel Sachet