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The present research work was broadly focused on the estimation of in Lacidipine in bulk and pharmaceutical dosage form by using two UV spectrophotometric methods like Zero order spectroscopy (Method-1), and first order derivative spectroscopy (Method-2). The solvent employed for the two methods was methanol and absorption maximum was found to be 284nm, and 277nm respectively. The developed methods showed linearity sin the range between 20-100µg/ml. The correlation co-efficient was ≥ 0.999. The precision (%RSD) for all the two methods was found to be ≤2. The accuracy was performed by spiking standard drug at 50,100 and 150% of the test concentration and the values obtained were within the limits. The assay for the formulation was found to be within limits. All the results were satisfactory the developed methods were linear, accurate, reproducible and robust.

Estimation of Lacidipine in Bulk and Pharmaceutical Dosage Form by Zero Order, and First Order Derivative UV Spectrophotometric Methods