Preparative Separation and Purification of Geniposide from Gardenia jasminoides Ellis fruit Using Macroporous Adsorption Resin D101

Gardenia fruit (Gardenia jasminoides Ellis), which are also known as the Fructus Gardenia, is widely distributed in Southeast Asia. In the Vietnamese and Chinese traditional medicine, Gardenia dried ripe fruit was used as an antiphlogistic, choleretic, diuretic, hemostatic and anticancer agent, and externally as a resolutive in treatment of sprains and bruises1. Phytochemical studies have been indicated that the principal bioactive compounds group in fruits are iridoid glycoside, in which geniposide is a main component with various biological activities such as anti-inflammatory, antidepressive, anti-angiogenic, and hepatic protection effects2, 3. In the past, the isolation techniques of geniposide from Gardenia fruit has been mainly performed by liquid-liquid extraction, absorption on activated charcoal4, silica gel column chromatography4, high-speed countercurrent chromatography5, and centrifugal partition chromatography6. However, these methods have several disadvantages such as time consumption, labor intensiveness, harmful and environmental pollution as well as very difficult to scale-up. Recently, macroporous resins have been applied in the purification and separation