The immune status of rabbits primed with chicken thymic protein

Chicken thymus glands were collected from three weeks old chicken through standard manual dissection technique .The glands were minced ,mortared ,centrifuged at 5000 RPM for 15 min .and supernatant was saved .Thymic protein were separated by PEG 6%( M.w 6000) solution .This protein is designed as chicken thymic protein (CTP). CTP was used a test immunogen and sensitizer .As an immunogen it was used in concentration ,1.1g/land 0.55 g/l for priming of rabbits using multi site injection protocol with Freunds complete adjuvant .Passive haemagglutination ,NBT ,LIF ,E- rosette and skin test were made on CTP primed rabbits and controls.It was found that CTP could acts as humoral immunogen ,T cell suppressor and LIF cytokine inducer. Thus CTP may contain lapin immunoregulatory protein